منابع مشابه
Measuring DNA repair capacity: small steps.
DNA repair capacity is a system of defenses designed to protect the integrity of the genome. The difficulty in studying DNA repair capacity in relationship to cancer etiology stems from the costs inherent in conducting whole cell assays, which represent an integrated response to DNA damage, and from the lack of specificity of the current genetic assays, which generally focus on the role of sing...
متن کاملDNA-Repair Capacity in Down\'s Syndrome
Down's syndrome (DS) is the most common chromosomal abnormality in human. Subjects with DS are known to be peridisposed to develop leukemia. The molecular basis of the association between DS and leukemia is unknown. The unscheduled DNA synthesis (UDS) test measure the ability of DNA-repair in mammalian cells after excision of a stretch of DNA containing the region of damage induced by chemical ...
متن کاملAn aphidicolin-block nucleotide excision repair assay measuring DNA incision and repair capacity.
The objective of the present study was to develop a cellular phenotype assay for nucleotide excision repair (NER), using benzo[a]pyrene diol epoxide (BPDE) as model mutagen. Since in vitro exposure to BPDE may lead to DNA strand breaks resulting from both direct interaction with DNA and incisions introduced by the repair enzymes, we aimed to discriminate between both types of breaks using the c...
متن کاملMeasuring oxidative DNA damage and DNA repair using the yeast comet assay.
Chromosomal DNA damage can be a result of several processes and agents of endogenous or exogenous origin. These cause strand breaks or oxidized bases that lead to strand breaks, which relax the normally supercoiled genomic DNA and increase its electrophoretic mobility. The extent of DNA damage can be assessed by single cell gel electrophoresis, where the chromosomal DNA migration distance corre...
متن کاملOGG1 DNA Repair Gene Polymorphism As a Biomarker of Oxidative and Genotoxic DNA Damage
Background: Single nucleotide polymorphisms in 8-oxoguanine DNA glycosylase-1 (OGG1) gene modulates DNA repair capacity and functions as one of the first lines of protective mechanisms against 8-hydroxy-2’-deoxyguanosine (8-OHdG) mutagenicity. OGG1-Cys326 gene polymorphism may decrease DNA repair function, causing oxidative stress due to higher oxidative DNA damage. The main purpose of this stu...
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ژورنال
عنوان ژورنال: Biotechnology & Biotechnological Equipment
سال: 2009
ISSN: 1310-2818,1314-3530
DOI: 10.1080/13102818.2009.10817632